Mycobacterium tuberculosisDNA Detection and Molecular Drug Susceptibility Test in AFB-stained Sputum Slides
نویسندگان
چکیده
منابع مشابه
Automatic Detection of Mycobacterium tuberculosis in Stained Sputum and Urine Smear Images
In this work we present an automatic algorithm for detection of mycobacterium tuberculosis from stained sputum smear images applying a novel method of tubeness filtering in conjunction with Otsu thresholding and connected component analysis. This eliminates human effort and measurement error from the detection of the bacteria, which normally requires a lab technician or pathologist to load a gl...
متن کاملTHE DETECTION OF Mycobacterium tuberculosis BY PCR IN SPUTUM SAMPLES FROM LONG-TERMTREATED LEPROSY PATIENTS: ASSOCIATIONS WITH SKIN TEST RESULTS AND IMMUNOTHERAPY WITH Mycobacterium vaccae
As part of a series of investigations at Baba Baghi Leprosarium in lran, 44 long-treated leprosy patients were selected for our study. Samples of early morning sputum were obtained from each patient, examined by microscopy for acid-fast bacilli (AFB), and cultured for tubercle bacilli. These tests were negative, but the polymerase chain reaction (PCR) for an insertion sequence believed to b...
متن کاملDirect detection of Mycobacterium tuberculosis rifampin resistance in bio-safe stained sputum smears
Direct smear microscopy of sputum forms the mainstay of TB diagnosis in resource-limited settings. Stained sputum smear slides can serve as a ready-made resource to transport sputum for molecular drug susceptibility testing. However, bio-safety is a major concern during transport of sputum/stained slides and for laboratory workers engaged in processing Mycobacterium tuberculosis infected sputum...
متن کاملDrug susceptibility test of Mycobacterium tuberculosis by nitrate reductase assay.
BACKGROUND Drug susceptibility testing for Mycobacterium tuberculosis (M. tuberculosis) is especially required in difficult cases of tuberculosis (TB) chemotherapy and in cases of multidrug resistance (MDR-TB; combined resistance to isonizid and rifampicin with or without resistance to any other drug). The methods for in vitro cultivation and drug susceptibility testing (DST) of M. tuberculosis...
متن کاملRapid detection of rifampicin susceptibility of Mycobacterium tuberculosis in sputum specimens by mycobacteriophage assay.
OBJECTIVE To evaluate the performance of FASTPlaqueTB-RIF, a newly introduced bacteriophage assay for rapid detection of rifampicin susceptibility of Mycobacterium tuberculosis in sputum specimens. METHODS A comparative study of 40 sputum specimens from patients of pulmonary tuberculosis, using FASTPlaqueTB-RIF and Bactec 460 TB system carried out at the Armed Forces Institute of Pathology, R...
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ژورنال
عنوان ژورنال: Biomedical Science Letters
سال: 2016
ISSN: 2288-7415
DOI: 10.15616/bsl.2016.22.1.24